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| Registros recuperados : 16 | |
| 2. |  | IGUMA, L. T.; SALES, J. N. S.; BATISTA, R. I. T. P.; PEREIRA, M. M.; QUINTAO, C. C. R.; VERNEQUE, R. da S.; CAMARGO, L. S. de A.; VIANA, J. H. M.; BARUSELLI, P. S. Evaluation of oocyte quality recovered from gir breed cows with synchronized follicular wave on in vitro embryo production. In: Annual Conference of the International Embryo Transfer Society, 2011, Orlando. Proceedings...| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | PEREIRA, M. M.; WOHLRES-VIANA, S.; SALES, J. N. S.; CAMARGO, A. R.; QUINTAO, C. C. R.; RABELO, N. C.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A.; GUIMARAES, M. F. M.; IGUMA, L. T. Differential gene expression od in vitro-matured bovine oocytes qith od without a polar body. In: ANNUAL CONFERENCE OF THE INTERNACIONAL EMBRYO TRANSFES SOCIETY, 2012, Phoenix. Proceedings... [S.l.:s.n.], 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | ANDRADE, J. P. N.; GOMEZ-LÉON, V. E.; ANDRADE, F. S.; CARVALHO, B. P.; LACOUTH, K. L.; GARCIA, F. Z.; JACOB, J. C. F.; SALES, J. N. S.; WILTBANK, M. C.; MELLO, M. R. B. Development of a novel 21-day reinsemination program, ReBreed21, in Bos indicus heifers. Theriogenology, v. 115, p. 125-131, Oct. 2020.| Biblioteca(s): Embrapa Acre. |
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| 5. | | SALES, J. N. S.; IGUMA, L. T.; QUINTAO, C. C. R.; GAMA, M. A. S. da; FREITAS, C. de; PEREIRA, M. M.; CAMARGO, L. S. de A.; VIANA, J. H. M.; BARUSELLI, P. S. Efeito da dieta com alta energia nos parâmetros metabólicos, endócrinos e reprodutivos de vacas Bos indicus e Bos taurus. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 395| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | SALES, J. N. S.; IGUMA, L. T.; BATISTA, R. I. T. P.; GAMA, M. A. S. da; CAMARGO, L. S. de A.; GIMENES, L. U.; MARQUES, M. G.; SIMOES, R.; FERNANDES, C. B.; VIANA, J. H. M.; BARUSELLI, P. S. Efeito da dieta com diferentes niveis de energia na quantidade e qualidade de oócitos de vacas não lactantes (Bos indicus e Bos taurus) submetidas a punção folicular. Acta Scientiae Veterinariae, v. 38, p. 385, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | SALES, J. N. S.; IGUMA, L. T.; BATISTA, R. I. T. P.; GAMA, M. A. S. da; CAMARGO, L. S. de A.; GIMENES, L. U.; MARQUES, M. G.; SIMOES, R.; FERNANDES, C. B.; VIANA, J. H. M.; BARUSELLI, P. S. Effect of different energy levels diet on oocyte quantity and quality of non lactating cows (Bos indicus and Bos taurus) submitted to ovum pick-up. Acta Scientiae Veterinariae, v. 38, p. s765, 2010. Supl.2.
Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | CAMARGO, L. S. de A.; SERAPIÃO, R. V.; QUINTAO, C. C. R.; PEREIRA, M. M.; OLIVEIRA, A. P.; SALES, J. N. S.; FREITAS, C. de; IGUMA, L. T.; VIANA, J. H. M. Produção de embriões clones bovinos a partir de oócitos obtidos de maturação in vivo. Acta Scientiae Veterinariae, v. 38, p. 402, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | QUINTAO, C. C. R.; VIANA, J. H. M.; SALES, J. N. S.; BATISTA, R. I. T. P.; PEREIRA, M. M.; OLIVEIRA, A. P.; CARVALHO, B. C.; CAMPOS JR. P. H.; SERAPIÃO, R. V.; FREITAS, C. de; CAMARGO, L. S. de A.; VERNEQUE, R. da S.; IGUMA, L. T. Comparação da taxa de prenhez inicial de embriões produzidos in vitro entre receptoras novilhas e vacas. Acta Scientiae Veterinariae, v. 38, p. 384, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | WOHLRES-VIANA, S.; PEREIRA, M. M.; SALES, J. N. S.; CAMARGO, J. DOS R.; QUINTAO, C. C. R.; RABELO, N. C.; DENICOL, A. C.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F.; IGUMA, L. T. Alteraçãoda expressão de genes relacionados com o metabolismo de glicose em oócitos bovinos maturados in vitro ou in vivo. In: Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 443| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | WOHLRES-VIANA, S.; PEREIRA, M. M.; SALES, J. N. S.; CAMARGO, A. J. DOS R.; QUINTAO, C. C. R.; RABELO, N. C.; DENICOL, A. C.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F.; IGUMA, L. T. Altered expression of genes related to glucose metabolism in bovine oocytes matured in vitro or in vivo. In: Annual Meeting SBTE, 25., 2011. Abstracts...| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | IGUMA, L. T.; PEREIRA, M. M.; WOHLRES-VIANA, S.; SALES, J. N. S.; QUINTAO, C. C. R.; PINTO, I. S. B.; SERAPIÃO, R. V.; BRITO, S. G.; CARAVALHO, B. C.; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F. Relative abundance of material transcripts in oocytes with and without polar body after in vitro maturation. In: Annual Meeting SBTE, 25., 2011. Abstracts...| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | SALES, J. N. S.; IGUMA, L. T.; BATISTA, R. I. T. P.; QUINTAO, C. C. R.; GAMA, M. A. S. da; FREITAS, C. de; PEREIRA, M. M.; CAMARGO, L. S. de A.; VIANA, J. H. M.; SOUZA, J. C.; BARUSELLI, P. S. Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows. Journal of Dairy Science, v. 98, n. 5, p. 3086-3099, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | SIMÕES, L. M. S.; SANTOS, A. P. C.; BOTTINO, M. P.; LIMA, E. A.; FERNANDES, U. R.; ORLANDI, R. E.; RODRIGUES, S. A. D.; CAIXETA, F. M.; ALVES, N. G.; SOUZA, J. C.; QUINTAO, C. C. R.; CAMARGO, L. S. A.; DODE, M. A. N.; SALES, J. N. S. Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles. Theriogenology, v. 166, p. 90-96, 2021.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | IGUMA, L. T.; PEREIRA, M. M.; VIANA, S. W.; SALES, J. N. S.; QUINTAO, C. C. R.; PINTO, I. S. B.; SERAPIÃO, R. V.; BRITO, S. G.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A.; MARTINS, M. F. Quantificação relativa de transcritos maternos em oocitos bovinos com e sem corpúsculo polar após maturação in vitro. In: Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 459| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | IGUMA, L. T.; BATISTA, R. I. T. P.; SALES, J. N. S.; PEREIRA, M. M.; PRATTI, S. M.; CARVALHO, B. C.; QUINTAO, C. C. R.; CAMPOS JR. P. H.; SERAPIÃO, R. V.; POLISSENI, J.; FREITAS, C. de; VERNEQUE, R. da S.; CAMARGO, L. S. de A.; VIANA, J. H. M.; BARUSELLI, P. S. In vitro production of embryos from Bos indicus and Bos taurus cows supplemented with two energy dietary levels. Acta Scientiae Veterinariae, v. 38, p. s756, 2010. Supl. 2.
Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 16 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/06/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SIMÕES, L. M. S.; SANTOS, A. P. C.; BOTTINO, M. P.; LIMA, E. A.; FERNANDES, U. R.; ORLANDI, R. E.; RODRIGUES, S. A. D.; CAIXETA, F. M.; ALVES, N. G.; SOUZA, J. C.; QUINTAO, C. C. R.; CAMARGO, L. S. A.; DODE, M. A. N.; SALES, J. N. S. |
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Afiliação: |
L. M. S. SIMÕES, UFLA; A. P. C. SANTOS, UFLA; M. P. BOTTINO, UFLA; E. A. LIMA, UFLA; U. R. FERNANDES, UFLA; R. E. ORLANDI, UFLA; S. A. D. RODRIGUES, UNB; F. M. CAIXETA, UNB; N. G. ALVES, UFLA; J. C. SOUZA, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; J. N. S. SALES, UFLA. |
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Título: |
Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 166, p. 90-96, 2021. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2020.12.021 |
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Idioma: |
Inglês |
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Conteúdo: |
The maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. MenosThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of met... Mostrar Tudo |
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Palavras-Chave: |
Fertilização in-vitro; IFOT; Intra-follicular. |
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Thesagro: |
Bovino; Fertilidade Animal. |
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Thesaurus NAL: |
Embryo (animal). |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03144naa a2200361 a 4500
001 2133325
005 2021-09-15
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2020.12.021$2DOI
100 1 $aSIMÕES, L. M. S.
245 $aNuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles.$h[electronic resource]
260 $c2021
520 $aThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability.
650 $aEmbryo (animal)
650 $aBovino
650 $aFertilidade Animal
653 $aFertilização in-vitro
653 $aIFOT
653 $aIntra-follicular
700 1 $aSANTOS, A. P. C.
700 1 $aBOTTINO, M. P.
700 1 $aLIMA, E. A.
700 1 $aFERNANDES, U. R.
700 1 $aORLANDI, R. E.
700 1 $aRODRIGUES, S. A. D.
700 1 $aCAIXETA, F. M.
700 1 $aALVES, N. G.
700 1 $aSOUZA, J. C.
700 1 $aQUINTAO, C. C. R.
700 1 $aCAMARGO, L. S. A.
700 1 $aDODE, M. A. N.
700 1 $aSALES, J. N. S.
773 $tTheriogenology$gv. 166, p. 90-96, 2021.
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